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Image Search Results
Journal: Scientific Reports
Article Title: SAS6-like protein in Plasmodium indicates that conoid-associated apical complex proteins persist in invasive stages within the mosquito vector
doi: 10.1038/srep28604
Figure Lengend Snippet: T. gondii expressing endogenous SAS6L with C-terminal fusion to HA-APEX fixed in host vacuoles and stained for HA (green) and either IMC1 (red, a and c ) or centrin (red, b ) ( a ) 3D-SIM super-resolution images with cell apices facing the viewer showing SAS6L rings (arrows, insets) and apices in profile showing SAS6L flush with the apical IMC. ( b ) Widefield images of parasites at different points of the cell cycle indicated by centrosome number and position (stained for centrin): interphase with single centrosome per cell (i); newly divided centrosomes close together (ii, upper vacuole); divided centrosomes migrated further apart (ii, lower vacuole; and iii); cells with nascent apical complexes (iv, arrowheads). ( c ) Widefield images of parasites showing daughter pellicle formation (stained for IMC1): before daughter pellicle formation (i and ii, upper vacuoles); small pellicle cups (i and ii, lower vacuoles; iii, upper vacuole); mid pellicle development (iii, lower vacuole). Scale bars = ( a ) 5 μm and 500 nm (inset); and ( b , c ) 10 μm.
Article Snippet: P. bergehi images were captured as described for live imaging using a 100x oil immersion objective, and T. gondii images were captured using a
Techniques: Expressing, Staining